Catalog No: OKEH00277
Size:96T
Price: $525.00
SKU
OKEH00277
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FABP4 ELISA Kit (Human) : 96 Wells (OKEH00277)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FABP4 ELISA Kit (Human) (OKEH00277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FABP4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for FABP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FABP4 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.4% (n = 20)
Mean Inter-assay CV%: < 10.5% (n = 20)
ELISA Kit Component
ComponentAmount
FABP4 Microplate96 Wells (12 x 8 Well strips)
FABP4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FABP4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Fatty acid-binding protein, adipocyte
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFABP4
Gene Full Namefatty acid binding protein 4
Alias Symbolsadipocyte fatty acid binding protein;adipocyte lipid-binding protein;adipocyte-type fatty acid-binding protein;AFABP;A-FABP;ALBP;aP2;epididymis secretory protein Li 104;fatty acid binding protein 4, adipocyte;Fatty acid-binding protein 4;fatty acid-binding protein, adipocyte;HEL-S-104.
NCBI Gene Id2167
Protein NameP15090
Description of TargetFatty acid-binding protein, adipocyte
Uniprot IDhttps://www.uniprot.org/uniprot/P15090
Protein Accession #NP_001433.1
Nucleotide Accession #NM_001442.2
Protein Size (# AA)132
Molecular Weight15 kDa
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