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F(ab')2 Antibody (OARA04020)

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Alias Symbols:
Goat F(ab')2 Anti-Human IgM Fc5u Antibody, Goat Fab2 Anti-Human IgM Fc5u Antibody
Description of Target:
F(ab')2 Anti-Human IgM Fc5u Antibody generated in goat detects specifically the Fc5u portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5u antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
F(ab')2 Human IgM Fc5u Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Anti-Human IgM Fc5u was produced by repeated immunization with human IgM Fc5µ fragment in goat.
Product Format:
Liquid. 0.02 M Postassium Phosphate, 0.15 M Sodium Chloride (pH 7.2) with 0.01% (w/v) Sodium azide.
Reconstitution and Storage:
Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid.   Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
1 mg/ml
Printable datasheet for OARA04020
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Application Info:
Human IgM Fc5u Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. ELISA: 1:20000
IHC: 1:1000~5000
WB: 1:2000~10000

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