Estrone ELISA Kit (OKEH02608)

Catalog No: OKEH02608

Size:96T

Estrone ELISA Kit (OKEH02608) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Estrone ELISA Kit (OKEH02608) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Estrone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Estrone and incubated. The Estrone found in the sample or standards competes with the biotinylated Estrone for limited binding sites on the immobilized anti-Estrone antibody. Excess unbound biotinylated Estrone and sample or standard Estrone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Estrone captured in the well and inversely proportional to the amount of Estrone which was contained in the sample or standard.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.5% (n = 20)
  Mean Inter-assay CV%: < 11.0% (n = 20)
• ELISA Kit Component:
  • Anti-Estrone Microplate: 96 Wells (12 x 8 Well strips)
  • Estrone Lyophilized Standard: 2 x 1,000 pg
  • Sample Diluent: 1 x 20 mL
  • 100X Estrone-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: E1
  Target Name: Estrone
  Pubchem: 5870
  Chemical formula: C₁₈H₂₂O₂
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 4.8 pg/mL
• Specificity: Natural and recombinant General Estrone
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: Estrone, E1, Oestrone
• Protein Name: Estrone
• Description of Target: Estrone is relevant to health and disease states because of its conversion to estrone sulfate, a long-lived derivative. Estrone sulfate acts as a reservoir that can be converted as needed to the more active estradiol. Estrone (E1, and also oestrone) is an estrogenic hormone secreted by the ovary as well as adipose tissue.