EPHB4 ELISA Kit (Human) (OKEH05438)

Catalog No: OKEH05438

Size:96T

Human EPHB4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology EPHB4 ELISA Kit (Human) (OKEH05438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHB4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPHB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPHB4 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 95%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.5%, n = 20
  Mean Inter-Assay Precision - <7.8%, n = 20
• ELISA Kit Component:
  • EPHB4 Microplate: 96 Wells (12 x 8 Well strips)
  • EPHB4 Lyophilized Standard: 2
  • 100X Biotinylated EPHB4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 8.04 pg/mL
• Specificity: Natural and recombinant Human Ephrin type-B receptor 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EPHB4
• Gene Full Name: EPH receptor B4
• Alias Symbols: CMAVM2;ephrin receptor EphB4;ephrin type-B receptor 4;hepatoma transmembrane kinase;HFASD;HTK;LMPHM7;MYK1;soluble EPHB4 variant 1;soluble EPHB4 variant 2;soluble EPHB4 variant 3;TYRO11;tyrosine-protein kinase receptor HTK;tyrosine-protein kinase TYRO11.
• NCBI Gene Id: 2050
• Protein Name: P54760
• Description of Target: Ephrin type-B receptor 4
• Uniprot ID: https://www.uniprot.org/uniprot/P54760
• Protein Accession #: NP_004435.3
• Nucleotide Accession #: NM_004444.4
• Protein Size (# AA): 987