Catalog No: OKAG00015 (Formerly GWB-SKR015)
Size:1 plate: 12 x 8-Well Microstrips
Price: $475.00
SKU
OKAG00015
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleThe Aviva Human Eotaxin-3 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hEotaxin-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Eotaxin-3 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range32-3,000 pg/mL
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, sera and plasma
Sensitivity32 pg/mL
SpecificityThe Human Eotaxin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Eotaxin-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: HCC-1, I-309, MCP-4, MIP-1α, MIP-1β, MIP-4, Eotaxin, Eotaxin-2, IL-20, RANTES, Resistin, sRANK-L
• Murine: Eotaxin, Eotaxin-2
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolCCL26
Gene Full NameC-C motif chemokine ligand 26
Alias SymbolsIMAC, TSC-1, MIP-4a, SCYA26, MIP-4alpha
NCBI Gene Id10344
Protein NameC-C motif chemokine 26
Description of TargetThis gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases.
Swissprot IdQ9Y258
Protein Accession #NP_006063.1
Nucleotide Accession #NM_006072.4
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CCL26.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CCL26.
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