ENO3 ELISA Kit (Mouse) (OKEH08747)

Catalog No: OKEH08747

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ENO3 ELISA Kit (Mouse) (OKEH08747) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENO3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ENO3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ENO3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.4% (n = 20)
  Mean Inter-assay CV%: <=7.6% (n = 20)
• ELISA Kit Component:
  • ENO3 Microplate: 96 Wells (12 x 8 Well strips)
  • ENO3 Lyophilized Standard: 2
  • 100X Biotinylated ENO3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Appears to have a function in striated muscle development and regeneration.
  Subcellular Location: Cytoplasm Localized to the Z line. Some colocalization with CKM at M-band (By similarity).
  Unigene: Mm.251322
  SMR: P21550
  String: 10090.ENSMUSP00000072620
  KEGG: mmu:13808
  Pfam: PF00113
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 36pg/mL
• Specificity: Natural and recombinant Mouse Beta-enolase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Eno3
• Gene Full Name: enolase 3, beta muscle
• Alias Symbols: 2-phospho-D-glycerate hydro-lyase;beta-enolase;Eno;Eno-3;Enolase 3;MSE;muscle-specific enolase;skeletal muscle enolase.
• NCBI Gene Id: 13808
• Protein Name: P21550
• Description of Target: Beta-enolase
• Uniprot ID: https://www.uniprot.org/uniprot/P21550
• Protein Accession #: NP_001129534.1
• Nucleotide Accession #: NM_001136062.2