ENHO High Sensitivity ELISA Kit (Human) (OKCD01388)

Catalog No: OKCD01388

Size:96WELLS

Human ENHO ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-105%	96-104%	91-105%	98-105%
  EDTA Plasma (n=5)	84-91%	82-103%	89-96%	79-97%
  Heparin Plasma (n=5)	99-105%	91-101%	95-102%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Adropin (AD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Adropin (AD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Adropin (AD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Adropin (AD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	97
  EDTA Plasma (n=5)	78-105	88
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Adropin (AD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Adropin (AD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ENHO Microplate: 96 Wells (12 x 8 Well Strips)
  • ENHO Lyophilized Standard: 2
  • 100X Biotinylated ENHO Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 4.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adropin (AD). No significant cross-reactivity or interference between Adropin (AD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ENHO
• Gene Full Name: energy homeostasis associated
• Alias Symbols: adropin;C9orf165;energy homeostasis-associated protein;GAAI470;UNQ470.
• NCBI Gene Id: 375704
• Protein Name: Adropin
• Description of Target: Involved in the regulation of glucose homeostasis and lipid metabolism.
• Uniprot ID: Q6UWT2
• Protein Accession #: NP_940975.2
• Nucleotide Accession #: NM_198573.2