ELOVL1 ELISA Kit (Human) (OKCD09153)

Catalog No: OKCD09153

Size:96WELLS

ELOVL1 ELISA Kit (Human) (OKCD09153) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ELOVL1 Microplate: 96 Wells (12 x 8 Well strips)
  • ELOVL1 Lyophilized Standard: 2
  • 100X ELOVL1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.062ng/mL

Target Info

• Gene Symbol: ELOVL1
• Gene Full Name: ELOVL fatty acid elongase 1
• Alias Symbols: 3-keto acyl-CoA synthase ELOVL1;CGI-88;elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1;elongation of very long chain fatty acids protein 1;ELOVL FA elongase 1;ELOVL fatty acid elongase 1;IKSHD;Ssc1;very long chain 3-ketoacyl-CoA synthase 1;very long chain 3-oxoacyl-CoA synthase 1.
• NCBI Gene Id: 64834
• Protein Name: Elongation of very long chain fatty acids protein 1
• Description of Target: Catalyzes the first and rate-limiting reaction of the four that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. Condensing enzyme that exhibits activity toward saturated C18 to C26 acyl-CoA substrates, with the highest activity towards C22:0 acyl-CoA. May participate in the production of both saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Important for saturated C24:0 and monounsaturated C24:1 sphingolipid synthesis. Indirectly inhibits RPE65 via production of VLCFAs.
• Uniprot ID: Q9BW60
• Protein Accession #: NP_001243328.1
• Nucleotide Accession #: NM_001256399.1