EFNB3 Antibody (OAAF01784)

Catalog No: OAAF01784 (Formerly GWB-ASD059)

Size:100 ug

Western blot analysis of extracts from SKOV3 cells, using EFNB3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EFNB3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EFNB3.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: EFNB3 antibody detects endogenous levels of total EFNB3 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Mitsutoshi Nakada, Cancer Res., Sep 2006; 66: 8492 - 8500.
  Klas Kullander, Genes & Dev., Apr 2001; 15: 877.
  M. Douglas Benson, PNAS, Jul 2005; 102: 10694 - 10699.
  Stephanie R. Kadison, J. Neurosci., Aug 2006; 26: 8909 - 8914.
• Publications:

  Estrogen and testosterone in concert with EFNB3 regulate vascular smooth muscle cell contractility and blood pressure. Am J Physiol Heart Circ Physiol. 310, H861-72 (2016). 26851246

• Description:
  View product citations for EFNB3 Antibody (OAAF01784) on CiteAb

Target Info

• Gene Symbol: EFNB3
• Gene Full Name: ephrin B3
• Alias Symbols: EFL6;EPH-related receptor transmembrane ligand ELK-L3;eph-related receptor tyrosine kinase ligand 8;ephrin-B3;EPLG8;LERK8.
• NCBI Gene Id: 1949
• Protein Name: Ephrin-B3
• Description of Target: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
• Uniprot ID: Q15768
• Protein Accession #: NP_001397.1
• Molecular Weight: 35 kDa