DUSP8 Antibody (OAGA00027)

Catalog No: OAGA00027

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:Raji
7.5% SDS PAGE
OAGA00027 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DUSP8(OAGA00027) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq]
  Positive Controls: Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1 and 44 of DUSP8 (Uniprot ID#Q13202)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.72 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DUSP8
• Gene Full Name: Dual specificity protein phosphatase 8
• Alias Symbols: HB5, HVH8, HVH-5, C11orf81
• NCBI Gene Id: 1850
• Protein Name: Dual specificity protein phosphatase 8
• Uniprot ID: Q13202
• Protein Accession #: NP_004411.2
• Molecular Weight: 66 kDa