DTX4 Antibody - N-terminal region (OAAB05466)

Catalog No: OAAB05466

Size:400UL

Western blot analysis of DTX4 Antibody (N-term) in HL-60 cell lysates (35ug/lane). DTX4 (arrow) was detected using the purified Pab.

DTX4 is strongly supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

DTX4 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DTX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human DTX4.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  DTX4 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: DTX4 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: DTX4
• Alias Symbols: AI449438, BC044798, D930042H13, deltex 4 homolog, deltex 4, E3 ubiquitin ligase, deltex homolog 4, deltex4, E3 ubiquitin-protein ligase DTX4, protein deltex-4, RING finger protein 155, RING-type E3 ubiquitin transferase DTX4, RNF1, RNF155.
• NCBI Gene Id: 207521|23220
• Protein Name: E3 ubiquitin-protein ligase DTX4|E3 ubiquitin-protein ligase DTX4 isoform 1 [Homo sapiens]
• Description of Target: Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4 (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.|Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.
• Uniprot ID: Q6PDK8|Q9Y2E6
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_055992.1
• Molecular Weight: 67258