DPPA2 ELISA Kit (Human) (OKEH08474)

Catalog No: OKEH08474

Size:96T

Human DPPA2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology DPPA2 ELISA Kit (Human) (OKEH08474) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPPA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPPA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPPA2 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.1% (n = 20)
  Mean Inter-assay CV%: <=4.6% (n = 20)
• ELISA Kit Component:
  • DPPA2 Microplate: 96 Wells (12 x 8 Well strips)
  • DPPA2 Lyophilized Standard: 2
  • 100X Biotinylated DPPA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Binds to target gene promoters, including NKX2-5 and SYCE1, but not GATA4, and may be involved in the maintenance of the active epigenetic status of these genes.
  Subcellular Location: Nucleus
  Unigene: Hs.351113
  SMR: Q7Z7J5
  String: 9606.ENSP00000417710
  KEGG: hsa:151871
  MIM: 614445
  Pfam: PF14047
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 40.5pg/mL
• Specificity: Natural and recombinant Human Developmental pluripotency-associated protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DPPA2
• Gene Full Name: developmental pluripotency associated 2
• Alias Symbols: cancer/testis antigen 100;CT100;developmental pluripotency-associated protein 2;ECAT15-2;embryonic stem cell (ESC) associated transcript 15-2;PESCRG1;pluripotent embryonic stem cell-related gene 1 protein.
• NCBI Gene Id: 151871
• Protein Name: Q7Z7J5
• Description of Target: Developmental pluripotency-associated protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q7Z7J5
• Protein Accession #: NP_620170.3
• Nucleotide Accession #: NM_138815.3