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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00146 |
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Predicted Species Reactivity | Human, Mouse, Fish, Zebrafish |
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Clonality | Monoclonal |
Clone | 4G11-C7 |
Isotype | IgG1 Kappa |
Host | Mouse |
Conjugation | Unconjugated |
Application | WB, IHC, IP, ChIP |
Additional Information | Background Info: It will cross-react with mouse DNMT1. |
:: | Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6). |
:: | Certificate of Analysis: 2 ug/mL was sufficient for detection of Dnmt1 in 10ug of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
Immunogen | Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1 |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Specificity | Detects ~180kDa. It will cross-react with mouse DNMT1. |
Dilution | WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user. |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Reference | 1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S. et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342. |
Description | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6). |
Gene Symbol | DNMT1 |
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Gene Full Name | DNA (cytosine-5-)-methyltransferase 1 |
Alias Symbols | DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI |
NCBI Gene Id | 1786 |
Uniprot ID | P26358 |
Protein Accession # | NP_001370 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "DNMT1 Antibody (OASE00146)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Fish, Zebrafish".
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How long will it take to receive "DNMT1 Antibody (OASE00146)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "DNMT1 Antibody (OASE00146)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "DNMT1 Antibody (OASE00146)"?
This target may also be called "DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI" in publications.
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What is the shipping cost for "DNMT1 Antibody (OASE00146)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "DNMT1 Antibody (OASE00146)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "DNMT1 Antibody (OASE00146)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "DNMT1 Antibody (OASE00146)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "DNMT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "DNMT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "DNMT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "DNMT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "DNMT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "DNMT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.