DKK3 ELISA Kit (Chicken) (OKEH08648)

Catalog No: OKEH08648

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology DKK3 ELISA Kit (Chicken) (OKEH08648) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DKK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DKK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DKK3 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.5% (n = 20)
  Mean Inter-assay CV%: <=7.8% (n = 20)
• ELISA Kit Component:
  • DKK3 Microplate: 96 Wells (12 x 8 Well strips)
  • DKK3 Lyophilized Standard: 2
  • 100X Biotinylated DKK3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  Subcellular Location: Secreted
  Unigene: Gga.3573
  SMR: Q90839
  String: 9031.ENSGALP00000008890
  KEGG: gga:396023
  Pfam: PF04706
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Dickkopf-related protein 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DKK3
• Gene Full Name: dickkopf WNT signaling pathway inhibitor 3
• Alias Symbols: dickkopf homolog 3;dickkopf-3;dickkopf-related protein 3;dkk-3;lens fiber protein CLFEST4.
• NCBI Gene Id: 396023
• Protein Name: Q90839
• Description of Target: Dickkopf-related protein 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q90839
• Protein Accession #: NP_990456.1
• Nucleotide Accession #: NM_205125.1