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Catalog No: OKEH02531
Size:96T
Price: $600.00
SKU
OKEH02531
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Dihydrotestosterone ELISA Kit (OKEH02531) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dihydrotestosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dihydrotestosterone and incubated. The Dihydrotestosterone found in the sample or standards competes with the biotinylated Dihydrotestosterone for limited binding sites on the immobilized anti-Dihydrotestosterone antibody. Excess unbound biotinylated Dihydrotestosterone and sample or standard Dihydrotestosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dihydrotestosterone captured in the well and inversely proportional to the amount of Dihydrotestosterone which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Dihydrotestosterone Microplate96 Wells (12 x 8 Well strips)
Dihydrotestosterone Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Dihydrotestosterone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: DHT
Target Name: Dihydrotestosterone
::Pubchem: 10635
::Chemical formula: C19H30O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.058 ng/mL
SpecificityNatural and recombinant General Dihydrotestosterone
Publications

Hair growth-promotion effects at the cellular level and antioxidant activity of the plant-based extract Phyllotexâ„¢. Heliyon. 7, e07888 (2021). 34522800

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsDHT, Dihydrotestosterone, DHT
Protein NameDihydrotestosterone
Description of TargetDihydrotestosterone (5alpha-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5alpha-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.
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