Dihydrotestosterone ELISA Kit (OKEH02531)

Catalog No: OKEH02531

Size:96 Wells

Dihydrotestosterone ELISA Kit (OKEH02531) standard curve.

Product Info

• Predicted Species Reactivity: All
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Dihydrotestosterone ELISA Kit (OKEH02531) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dihydrotestosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dihydrotestosterone and incubated. The Dihydrotestosterone found in the sample or standards competes with the biotinylated Dihydrotestosterone for limited binding sites on the immobilized anti-Dihydrotestosterone antibody. Excess unbound biotinylated Dihydrotestosterone and sample or standard Dihydrotestosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dihydrotestosterone captured in the well and inversely proportional to the amount of Dihydrotestosterone which was contained in the sample or standard.
• ELISA Kit Range: 0.156 - 10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 7.8% (n = 20)
• ELISA Kit Component:
  • Anti-Dihydrotestosterone Microplate: 96 Wells (12 x 8 Well strips)
  • Dihydrotestosterone Lyophilized Standard: 2 x 10 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Dihydrotestosterone-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: DHT
  Target Name: Dihydrotestosterone
  Pubchem: 10635
  Chemical formula: C19H30O2
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.058 ng/mL
• Specificity: Natural and recombinant General Dihydrotestosterone
• Publications:

  Hair growth-promotion effects at the cellular level and antioxidant activity of the plant-based extract Phyllotexâ„¢. Heliyon. 7, e07888 (2021). 34522800

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: DHT, Dihydrotestosterone, DHT
• Protein Name: Dihydrotestosterone
• Description of Target: Dihydrotestosterone (5alpha-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5alpha-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.