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Dihydrotestosterone ELISA Kit (OKEH02531)

96 Wells
$559.00
In Stock
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Alias Symbols:
DHT, Dihydrotestosterone, DHT
Description of Target:
Dihydrotestosterone (5alpha-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5alpha-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.
Protein Name:
Dihydrotestosterone
Predicted Species Reactivity:
All
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity:
< 0.056 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Dihydrotestosterone ELISA Kit (OKEH02531) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dihydrotestosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dihydrotestosterone and incubated. The Dihydrotestosterone found in the sample or standards competes with the biotinylated Dihydrotestosterone for limited binding sites on the immobilized anti-Dihydrotestosterone antibody. Excess unbound biotinylated Dihydrotestosterone and sample or standard Dihydrotestosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dihydrotestosterone captured in the well and inversely proportional to the amount of Dihydrotestosterone which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-Dihydrotestosterone Microplate96 Wells (12 x 8 Well strips)
Dihydrotestosterone Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Dihydrotestosterone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into Serum and Plasma = 96%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Competitive ELISA
Additional Information:
Target Abbr: DHT
Target Name: Dihydrotestosterone
:::
Pubchem: 10635
:::
Chemical formula: C19H30O2

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