DIAPH2 Antibody - middle region (OAAB06000)

Catalog No: OAAB06000

Size:400UL

Western blot analysis of DIAPH2 Antibody (Center) in 293, MDA-MB435 cell lysates (35ug/lane). DIAPH2 (arrow) was detected using the purified Pab.

There is BioGPS gene expression data showing that DIAPH2 is expressed in HEK293, MDA-MB435

Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

DIAPH2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 amino acids from the Central region of human DIAPH2.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that DIAPH2 is expressed in HEK293, MDA-MB435

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: DIAPH2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: DIAPH2
• Alias Symbols: DIA, DIA2, diaphanous homolog 2, Diaphanous-related formin-2, diaphorase-2, DRF2, POF, POF2, POF2A, protein diaphanous homolog 2.
• NCBI Gene Id: 1730
• Protein Name: Protein diaphanous homolog 2|protein diaphanous homolog 2 isoform 12C [Homo sapiens]|protein diaphanous homolog 2 isoform 156 [Homo sapiens]
• Description of Target: Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
• Uniprot ID: O60879
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_006720.1,NP_009293.1
• Molecular Weight: 125569