Catalog No: OKEH04268
Size:96 Wells
Price: $525.00
SKU
OKEH04268
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DEXI ELISA Kit (Human) (OKEH04268) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEXI has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEXI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEXI captured in the well.
ELISA Kit Range0.78 - 50ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.5% (n = 20)
Mean Inter-assay CV%: <= 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
DEXI Microplate96 Wells (12 x 8 Well Strips)
DEXI Standard2 x 2 ng
Sample Diluent1 x 20 mL
DEXI Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.34 ng/mL
SpecificityNatural and recombinant Human Dexamethasone-induced protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDEXI
Gene Full NameDexi homolog
Alias SymbolsMYLE
NCBI Gene Id28955
Protein Namedexamethasone-induced protein
Swissprot IdO95424
Protein Accession #NP_054734.2
Nucleotide Accession #NM_014015.3
Protein Size (# AA)95
Molecular Weight10 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express DEXI.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express DEXI.
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