DENND1B Antibody (OAGA00563)

Catalog No: OAGA00563

Size:100 ul

Sample (30 ug of whole cell lysate)
A: MOLT4
7.5% SDS PAGE
OAGA00563 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00563 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B(OAGA00563) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 272 of DENND1B (Uniprot ID#Q6P3S1)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.9 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DENND1B
• Gene Full Name: DENN domain-containing protein 1B
• Alias Symbols: FAM31B, C1ORF18, C1orf218
• NCBI Gene Id: 163486
• Protein Name: DENN domain-containing protein 1B
• Uniprot ID: Q6P3S1
• Protein Accession #: NP_001182144.1
• Molecular Weight: 48 kDa