DEFB105A ELISA Kit (Human) (OKWB00227)

Catalog No: OKWB00227

Size:96T

Human DEFB105A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-104%	85-101%	92-103%	89-98%
  EDTA Plasma (n=5)	82-89%	83-99%	86-101%	82-100%
  Heparin Plasma (n=5)	94-100%	81-100%	81-99%	88-100%

• ELISA Kit Principle: Aviva Systems Biology DEFB105A ELISA Kit (Human) (OKWB00227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB105A has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEFB105A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB105A captured in the well.
• ELISA Kit Range: 78.125-5000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	93-105	98
  EDTA Plasma (n=5)	86-105	96
  Heparin Plasma (n=5)	90-101	96

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • DEFB105A Microplate: 96 Wells (12 x 8 Well strips)
  • DEFB105A Lyophilized Standard: 2 x 5 ng
  • 100X DEFB105A Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 46.9 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DEFB105A|DEFB105B
• Gene Full Name: defensin beta 105A|defensin beta 105B
• Alias Symbols: BD-5;beta defensin 5;beta-defensin 105;beta-defensin 5;DEFB105;DEFB-5;defensin, beta 105;defensin, beta 5.
• NCBI Gene Id: 245908|504180
• Protein Name: Beta-defensin 105
• Description of Target: Has antibacterial activity.
• Uniprot ID: Q8NG35
• Protein Accession #: NP_001035793
• Nucleotide Accession #: NM_001040703