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Datasheets/Manuals | Printable datasheet for DEFB1 Polyclonal Antibody (OABB00404) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: DEFB1,Beta-defensin 1, is a protein that in humans is encoded by the DEFB1 gene. DEFB1 belongs to the defensin family. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. The DEFB1 gene spans more than 7 kb and includes a large 6,962-bp intron. By 2-color fluorescence in situ hybridization on both metaphase chromosomes and released chromatin fiber, the DEFB1gene is mapped to 8p23.2-p23.1, within 100 to 150 kb of the gene for human neutrophil defensin-alpha-1. DEFB1may augment neonatal host defenses through antimicrobial effects or prime the adaptive immune system at mucosal surfaces. |
:: | Background: DEFB1,Beta-defensin 1, is a protein that in humans is encoded by the DEFB1 gene. DEFB1 belongs to the defensin family. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. The DEFB1 gene spans more than 7 kb and includes a large 6,962-bp intron.By 2-color fluorescence in situ hybridization on both metaphase chromosomes and released chromatin fiber, the DEFB1gene is mapped to 8p23.2-p23.1, within 100 to 150 kb of the gene for human neutrophil defensin-alpha-1. DEFB1may augment neonatal host defenses through antimicrobial effects or prime the adaptive immune system at mucosal surfaces. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Reference | 1. Jia, H. P., Starner, T., Ackermann, M., Kirby, P., Tack, B. F., McCray, P. B., Jr.Abundant human beta-defensin-1 expression in milk and mammary gland epithelium.J. 2. Liu, L., Zhao, C., Heng, H. H. Q., Ganz, T.The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two peptide families with differing disulfide topology share a common ancestry. 3. Valore, E. V., Park, C. H., Quayle, A. J., Wiles, K. R., McCray, P. B., Jr., Ganz, T.Human beta-defensin-1: an antimicrobial peptide of urogenital tissues.J. Clin. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Beta-defensin 1(DEFB1) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | DEFB1 |
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Gene Full Name | defensin beta 1 |
Alias Symbols | BD1;BD-1;beta-defensin 1;DEFB-1;DEFB101;Defensin, beta 1;HBD1. |
NCBI Gene Id | 1672 |
Protein Name | Beta-defensin 1 |
Description of Target | Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636). |
Uniprot ID | P60022 |
Molecular Weight | 7420 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "DEFB1 Polyclonal Antibody (OABB00404)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "DEFB1 Polyclonal Antibody (OABB00404)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "DEFB1 Polyclonal Antibody (OABB00404)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "DEFB1 Polyclonal Antibody (OABB00404)"?
This target may also be called "BD1;BD-1;beta-defensin 1;DEFB-1;DEFB101;Defensin, beta 1;HBD1." in publications.
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What is the shipping cost for "DEFB1 Polyclonal Antibody (OABB00404)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "DEFB1 Polyclonal Antibody (OABB00404)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "DEFB1 Polyclonal Antibody (OABB00404)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "7420 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "DEFB1 Polyclonal Antibody (OABB00404)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "DEFB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "DEFB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "DEFB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "DEFB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "DEFB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "DEFB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.