DDOST ELISA Kit (Human) (OKCD09177)

Catalog No: OKCD09177

Size:96WELLS

DDOST ELISA Kit (Human) (OKCD09177) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DDOST Microplate: 96 Wells (12 x 8 Well strips)
  • DDOST Lyophilized Standard: 2
  • 100X DDOST HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.126ng/mL

Target Info

• Gene Symbol: DDOST
• Gene Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
• Alias Symbols: advanced glycation endproduct receptor 1;advanced glycation end-product receptor 1;AGER1;CDG1R;dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit;dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic);dolichyl-diphosphooligosaccharide-protein glycotransferase;GATD6;OKSWcl45;oligosaccharyl transferase 48 kDa subunit;oligosaccharyltransferase 48 kDa subunit;oligosaccharyltransferase subunit 48;OST;OST48;WBP1.
• NCBI Gene Id: 1650
• Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
• Description of Target: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.
• Uniprot ID: P39656
• Protein Accession #: NP_005207.2
• Nucleotide Accession #: NM_005216.4