DDAH2 ELISA Kit (Human) (OKCD08580)

Catalog No: OKCD08580

Size:96WELLS

DDAH2 ELISA Kit (Human) (OKCD08580) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DDAH2 Microplate: 96 Wells (12 x 8 Well strips)
  • DDAH2 Lyophilized Standard: 2
  • 100X DDAH2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.116ng/mL

Target Info

• Gene Symbol: DDAH2
• Gene Full Name: dimethylarginine dimethylaminohydrolase 2
• Alias Symbols: DDAH;DDAHII;dimethylargininase-2;dimethylarginine dimethylaminohydrolase II;epididymis secretory protein Li 277;G6a;HEL-S-277;N(G),N(G)-dimethylarginine dimethylaminohydrolase 2;NG30;Protein G6a;S-phase protein;testis tissue sperm-binding protein Li 54e.
• NCBI Gene Id: 23564
• Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
• Description of Target: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
• Uniprot ID: O95865
• Protein Accession #: NP_001289936.1
• Nucleotide Accession #: NM_001303007.1