DAXX ELISA Kit (Dog) (OKEH08173)

Catalog No: OKEH08173

Size:96T

Dog DAXX ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	101-111% (n = 20)	108-116% (n = 20)	106-116% (n = 20)	105-114% (n = 20)
  EDTA plasma(n=5)	87-97% (n = 20)	100-113% (n = 20)	93-105% (n = 20)	98-108% (n = 20)
  heparin plasma(n=5)	102-114% (n = 20)	90-99% (n = 20)	90-100% (n = 20)	90-102% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology DAXX ELISA Kit (Dog) (OKEH08173) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DAXX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DAXX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DAXX captured in well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.1% (n = 20)
  Mean Inter-assay CV%: <=4.4% (n = 20)
• ELISA Kit Component:
  • DAXX Microplate: 96 Wells (12 x 8 Well strips)
  • DAXX Lyophilized Standard: 2
  • 100X Biotinylated DAXX Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional corepressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
  Subcellular Location: Cytoplasm Nucleus nucleoplasm Nucleus PML body Nucleus nucleolus Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures.
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 21pg/ml
• Specificity: Natural and recombinant Dog Death domain-associated protein 6
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DAXX
• Alias Symbols: Daxx.
• Protein Name: Q5TJE1
• Description of Target: Death domain-associated protein 6
• Uniprot ID: https://www.uniprot.org/uniprot/Q5TJE1