DAPK3 Antibody (OAAB18357)

Catalog No: OAAB18357

Size:100ul

Western blot analysis of lysates from Hela, HepG2 cell line (from left to right), using DAPK3 Antibody (Ab-265) (B0900).
B0900 was diluted at 1:1000 at each lane.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.
Dilution: 1:1000

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using DAPK3 (Ab-265) antibody.
Dilution: 1:50-1:100

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IHC: 1:50~1:100
• Reference: Kawai T., et al.Mol. Cell. Biol. 18:1642-1651(1998).
  Murata-Hori M., et al.FEBS Lett. 451:81-84(1999).
  Ota T., et al.Nat. Genet. 36:40-45(2004).
  Takamoto N., et al.Arch. Biochem. Biophys. 456:194-203(2006).
  Preuss U., et al.Eur. J. Cell Biol. 82:447-459(2003).

Target Info

• Gene Symbol: DAPK3
• Alias Symbols: DLK, ZIP, ZIPK
• NCBI Gene Id: 1613
• Description of Target: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, its association with the GAIT complex and its subsequent involvement in transcript- selective translation inhibition.
• Uniprot ID: O43293
• Molecular Weight: 53 kDa