D130043K22Rik Antibody (OAEB01145)

Catalog No: OAEB01145 (Formerly GWB-EV0054)

Size:100UG

OAEB01145 (0.5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: D130043K22Rik
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Mouse: 100%
• Peptide Sequence: C-QGKIKQENKPTLH
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 150kDa band observed in Mouse Brain lysates (calculated MW of 118kDa according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17(6):859-71; PMID: 18063668). Recommended concentration: 0.5-1.5ug/ml.
  An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Paracchini S, Thomas A, Castro S, Lai C, Paramasivam M, Wang Y, Keating BJ,
  Taylor JM, Hacking DF, Scerri T, Francks C, Richardson AJ, Wade-Martins R, Stein
  JF, Knight JC, Copp AJ, Loturco J, Monaco AP.; The chromosome 6p22 haplotype associated with dyslexia reduces the expression of KIAA0319, a novel gene involved in neuronal migration.; Hum Mol Genet. 2006 May 15;15(10):1659-66.; Pubmed 16600991

Target Info

• Gene Symbol: D130043K22Rik|RGD1307443
• Gene Full Name: RIKEN cDNA D130043K22 gene|similar to mKIAA0319 protein
• Alias Symbols: 4930451E12Rik;dyslexia susceptibility 2;dyslexia-associated protein KIAA0319 homolog;Kiaa0319.
• NCBI Gene Id: 210108|361244
• Protein Name: Dyslexia-associated protein KIAA0319 homolog
• Uniprot ID: Q5SZV5
• Protein Accession #: NP_001074520.1
• Molecular Weight: 118