CYP2A6 ELISA Kit (Human) (OKEI00322)

Catalog No: OKEI00322

Size:96 Wells

Human CYP2A6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CYP2A6 ELISA Kit (Human) (OKEI00322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP2A6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP2A6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CYP2A6
• ELISA Kit Range: 1.563 - 100 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CYP2A6. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CYP2A6 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CYP2A6 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP2A6 Lyophilized Standard: 2
  • 100X CYP2A6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 0.938 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP2A6
• Alias Symbols: CPA6, CYP2A, CYP2A3, P450PB, CYPIIA6, P450C2A
• NCBI Gene Id: 1548
• Protein Name: Cytochrome P450 2A6
• Description of Target: Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
• Uniprot ID: P11509
• Protein Accession #: NP_000753
• Nucleotide Accession #: NM_000762