Catalog No: OKEI00322
Size:96 Wells
Price: $625.00
SKU
OKEI00322
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP2A6 ELISA Kit (Human) (OKEI00322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP2A6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP2A6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CYP2A6
ELISA Kit Range1.563 - 100 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CYP2A6. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CYP2A6 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CYP2A6 Microplate96 Wells (12 x 8 Well strips)
CYP2A6 Lyophilized Standard2
100X CYP2A6 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.938 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYP2A6
Alias SymbolsCPA6, CYP2A, CYP2A3, P450PB, CYPIIA6, P450C2A
NCBI Gene Id1548
Protein NameCytochrome P450 2A6
Description of TargetExhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
Swissprot IdP11509
Protein Accession #NP_000753
Nucleotide Accession #NM_000762
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CYP2A6.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CYP2A6.
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