CXCL13 Chemi-Luminescent ELISA Kit (Human) (OKCD03319)

Catalog No: OKCD03319

Size:96WELLS

Human CXCL13 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-99%	87-95%	87-101%	88-97%
  EDTA Plasma (n=5)	82-98%	85-94%	95-104%	93-105%
  Heparin Plasma (n=5)	94-105%	80-101%	95-104%	83-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant (BLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant (BLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Lymphocyte Chemoattractant (BLC) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-94	91
  EDTA Plasma (n=5)	78-91	88
  Heparin Plasma (n=5)	89-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL13 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL13 Lyophilized Standard: 2
  • 100X CXCL13 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL13
• Gene Full Name: C-X-C motif chemokine ligand 13
• Alias Symbols: ANGIE;ANGIE2;b cell-attracting chemokine 1;b lymphocyte chemoattractant;BCA1;BCA-1;B-cell chemoattractant;B-cell-attracting chemokine 1;B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1);BLC;BLR1L;B-lymphocyte chemoattractant;chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant);CXC chemokine BLC;C-X-C motif chemokine 13;SCYB13;small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant);small-inducible cytokine B13.
• NCBI Gene Id: 10563
• Protein Name: C-X-C motif chemokine
• Description of Target: C-X-C motif chemokine
• Uniprot ID: Q53X90
• Protein Accession #: NP_006410
• Nucleotide Accession #: NM_006419