CSK Antibody - N-terminal region (OAAB10048)

Catalog No: OAAB10048

Size:400ul

CSK Antibody (N-term) (Cat. #OAAB10048) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CSK antibody detected the CSK protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
  Function: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
  Cellular Location: Cytoplasm. Cell membrane. Note=Mainly cytoplasmic, also present in lipid rafts.
  Tissue Location: Expressed in lung and macrophages.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human CSK.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Chicken, Mouse, Rat
• Concentration: 0.6 mg/ml
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: CSK
• Alias Symbols: CSK, Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL
• NCBI Gene Id: 1445
• Protein Name: Tyrosine-protein kinase CSK
• Description of Target: Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
• Uniprot ID: P41240
• Protein Accession #: NP_001120662.1
• Molecular Weight: 50 kDa