CSF1R/M-CSFR ELISA Kit (Mouse) (OKBB00441)

Catalog No: OKBB00441

Size:96 Wells

Mouse CSF1R/M-CSFR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CSF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CSF1R has been precoated onto 96-well plates. Standards(NS0, A20-S511) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CSF1R amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	379	1361	2468	413	1533	2766
  SD	20.85	62.61	155.5	28.5	89	202
  CV%	5.5	4.6	6.3	6.9	5.8	7.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse M-CSFR Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse M-CSFR Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse M-CSFR Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CSF1R
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A20-S511

Target Info

• Gene Symbol: CSF1R
• Gene Full Name: colony stimulating factor 1 receptor
• Alias Symbols: C FMS, C FMS, CD 115, CD115, CD115 antigen, CFMS, Colony stimulating factor 1 receptor, CSF 1 R, CSF 1R, CSF-1 receptor, CSF-1-R, CSF1 R, CSF1R, CSF1R_HUMAN, CSFR, FIM 2, FIM2, FMS, FMS proto oncogene, FMS protooncogene, HDLS, M-CSF Receptor, M-CSF-R, Macrophage colony stimulating factor 1 receptor, Macrophage colony stimulating factor I receptor, Macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v fms) oncogene homolog, Oncogen FMS, Proto-oncogene c-Fms, V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
• NCBI Gene Id: 12978
• Protein Name: Macrophage colony-stimulating factor 1 receptor
• Description of Target: CSF1R (Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
• Uniprot ID: P09581
• Protein Accession #: NP_001032948.2
• Nucleotide Accession #: NM_001037859.2