CRTAP ELISA Kit (Mouse) (OKCD09190)

Catalog No: OKCD09190

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cartilage Associated Protein (CRTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cartilage Associated Protein (CRTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cartilage Associated Protein (CRTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cartilage Associated Protein (CRTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cartilage Associated Protein (CRTAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cartilage Associated Protein (CRTAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CRTAP Microplate: 96 Wells (12 x 8 Well strips)
  • CRTAP Lyophilized Standard: 2
  • 100X CRTAP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: Crtap
• Gene Full Name: cartilage associated protein
• Alias Symbols: 5730529N23Rik;C;cartilage-associated protein;CASP;Lep;Leprel3;P3h;P3h5.
• NCBI Gene Id: 56693
• Protein Name: Cartilage-associated protein
• Description of Target: Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.
• Uniprot ID: Q9CYD3
• Protein Accession #: NP_064306.2
• Nucleotide Accession #: NM_019922.2