CR2 Antibody (OAEE00727)

Catalog No: OAEE00727

Size:100T

Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD21 (LT21) antibody.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Porcine
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: LT21
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: IM9 human B-lymphoblastoid cell line
• Purification: Purified by size-exclusion chromatography
• Concentration: 20 ul / test
• Specificity: The antibody LT21 reacts with CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.
  HLDA VI; WS Code B CD21.1
• Formulation: FITC
• Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
  Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62.
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3
  Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization andpathogenesis. J Immunol. 2007 Nov 1;179(9):6144-52.
  Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45(3):169-79.
  Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44(13):3434-44.
  Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66.

Target Info

• Gene Symbol: CR2
• Gene Full Name: complement C3d receptor 2
• Alias Symbols: C3DR;CD21;Complement C3d receptor;complement component (3d/Epstein Barr virus) receptor 2;complement component 3d receptor 2;complement receptor type 2;CR;CVID7;EBV receptor;Epstein-Barr virus receptor;SLEB9.
• NCBI Gene Id: 1380
• Protein Name: Complement receptor type 2
• Description of Target: Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).
• Uniprot ID: P20023
• Protein Accession #: NP_001006659.1; NP_001868.2
• Nucleotide Accession #: NM_001006658.2; NM_001877.4