CPEB1 Antibody (OAPB00712)

Catalog No: OAPB00712

Size:100UG

Western blot analysis of CPEB1 in rat brain tissue lysate with CPEB1 antibody at (A) 1 and (B) 2 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. CPEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Western blot
  Positive Control: Rat brain tissue lysate (oapb00712)
• Reconstitution and Storage: Can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: CPEB1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human CPEB1. The immunogen is located within amino acids 50 - 100 of CPEB1.
• Purification: Affinity Purified
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CPEB1 (OAPB00712) antibody is Catalog # OEPB02830
• Application Info: WB: 1-2 ug/mL.
  Optimal dilutions should be determined by the end user.
• Reference: Richter JD. CPEB: a life in translation. Trends in Biochem. Sci.2007; 32:179-85.;
  Jones KJ, Korb E, Kundel MA, et al. CPEB1 regulates beta-catenin mRNA translation and cell migration in astrocytes. Glia2008; 56:1401-13. ;
  Hagele S, Kuhn U, Boning M, et al. Cytoplasmic polyadenylation element binding protein (CPEB)1 and 2 bind to the HIF-1alpha mRNA 3'UTR and modulate HIF-1 alpha protein expression. Biochem. J.2008; epub.;

Target Info

• Gene Symbol: CPEB1
• Gene Full Name: cytoplasmic polyadenylation element binding protein 1
• Alias Symbols: CPEB;CPEB-1;CPE-binding protein 1;CPE-BP1;cytoplasmic polyadenylation element-binding protein 1;h-CPEB;hCPEB-1.
• NCBI Gene Id: 64506
• Protein Name: Cytoplasmic polyadenylation element-binding protein 1
• Description of Target: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed:26398195).
• Uniprot ID: Q9BZB8
• Protein Accession #: NP_001073001.1; NP_001073002.1; NP_001073003.1; NP_001275748.1; NP_085097.3
• Nucleotide Accession #: NM_001079533.1; NM_001079534.1; NM_001079535.1; NM_001288819.1; NM_030594.4