COX5B Antibody (OAGA00096)

Catalog No: OAGA00096

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:Hep G2
15% SDS PAGE
OAGA00096 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using COX5B(OAGA00096) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq]
  Positive Controls: HepG2
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 129 of COX5B (Uniprot ID#P10606)
• Purification: Affinity purified by Protein A.
• Concentration: 1.00 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: COX5B
• Gene Full Name: Cytochrome c oxidase subunit 5B, mitochondrial
• Alias Symbols: COXVB
• NCBI Gene Id: 1329
• Protein Name: Cytochrome c oxidase subunit 5B, mitochondrial
• Uniprot ID: P10606
• Protein Accession #: NP_001853.2
• Molecular Weight: 14 kDa