COL6A3 ELISA Kit (Human) (OKCD04390)

Catalog No: OKCD04390

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-102%	86-98%	83-105%	78-91%
  EDTA Plasma (n=5)	92-105%	80-101%	79-99%	79-97%
  Heparin Plasma (n=5)	90-102%	78-104%	80-91%	81-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VI Alpha 3 (COL6a3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type VI Alpha 3 (COL6a3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VI Alpha 3 (COL6a3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type VI Alpha 3 (COL6a3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	90-99	95
  Heparin Plasma (n=5)	93-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type VI Alpha 3 (COL6a3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type VI Alpha 3 (COL6a3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • COL6a3 Microplate: 96 Wells (12 x 8 Well strips)
  • COL6a3 Lyophilized Standard: 2
  • 100X COL6a3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 50 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COL6A3
• Gene Full Name: collagen type VI alpha 3 chain
• Alias Symbols: BTHLM1;collagen alpha-3(VI) chain;collagen VI, alpha-3 polypeptide;collagen, type VI, alpha 3;DYT27;epididymis secretory sperm binding protein;UCMD1.
• NCBI Gene Id: 1293
• Protein Name: Collagen alpha-3(VI) chain
• Description of Target: Collagen VI acts as a cell-binding protein.
• Uniprot ID: P12111
• Protein Accession #: NP_004360.2
• Nucleotide Accession #: NM_004369.3