CNTN1 Antibody - middle region (OAEB03211)

Catalog No: OAEB03211

Size:100UG

OAEB03211 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB03211 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC-FFPE
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CNTN1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-QVTSQEYSARLEN
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize isoform 1 (NP_001834.2) and isoform 2 (NP_778203.1).
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western blot: Approx 150kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 113kDa according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3ug/ml.
  IHC: In paraffin embedded Human Cerebellum shows axonal staining in the molecular layer near the granular layer. Recommended concentration, 5-10 ug/ml.

Target Info

• Gene Symbol: Cntn1|CNTN1
• Gene Full Name: contactin 1
• Alias Symbols: AW495098;CN;CNTN;contactin-1;F3;F3ca;F3cam;glycoprotein gP135;GP135;MYPCN;neural cell surface protein F3;u;usl.
• NCBI Gene Id: 117258|1272|12805
• Protein Accession #: NP_001834.2;
  NP_778203.1
• Molecular Weight: 113, 112