CLIC4 Antibody (OAAF03488)

Catalog No: OAAF03488 (Formerly GWB-ASE474)

Size:100 ug

Western blot analysis of extracts from HT-29 cells, using CLIC4 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using CLIC4 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CLIC4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: CLIC4 antibody detects endogenous levels of total CLIC4 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Wiemann S., Genome Res. 11:422-435(2001).
  Edwards J.C.; Am. J. Physiol. 276:F398-F408(1999).
  Chuang J.Z., J. Neurosci. 19:2919-2928(1999).

Target Info

• Gene Symbol: CLIC4
• Gene Full Name: chloride intracellular channel 4
• Alias Symbols: chloride intracellular channel 4 like;chloride intracellular channel protein 4;CLIC4L;epididymis secretory sperm binding protein;H1;huH1;intracellular chloride ion channel protein p64H1;MTCLIC;p64H1.
• NCBI Gene Id: 25932
• Protein Name: Chloride intracellular channel protein 4
• Description of Target: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
• Uniprot ID: Q9Y696
• Protein Accession #: NP_039234.1
• Molecular Weight: 28 kDa