Catalog No: OKCD07926
Size:96 Wells
Price: $825.00
SKU
OKCD07926
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CKB ELISA Kit (Mouse) (OKCD07926)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CKB ELISA Kit (Mouse) (OKCD07926) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CKB captured in the well.
ELISA Kit Range78-5,000pg/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CKB. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CKB using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CKB Microplate96 Wells (12 x 8 Well strips)
CKB Lyophilized Standard2
100X CKB HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 30pg/mL
Gene SymbolCKB
Gene Full Namecreatine kinase B
Alias SymbolsBck, Ck3, B-CK, Ck-3, Ckbb, CPK-B
NCBI Gene Id12709
Protein Namecreatine kinase B-type
Description of TargetReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Swissprot IdQ04447
Protein Accession #NP_067248.1
Nucleotide Accession #NM_021273.4
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CKB.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CKB.
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