CIAP2 Antibody (OABB02010)

Catalog No: OABB02010

Size:100UG

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Anti-cIAP2 Picoband antibody, OABB02010, Western blotting
All lanes: Anti cIAP2 (OABB02010) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: Baculoviral IAP repeat-containing protein 3 (also known as cIAP2) is a protein that in humans is encoded by the BIRC3 gene. cIAP2 is a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human cIAP2 recombinant protein (Position: M1-K191). Human cIAP2 shares 69.8% amino acid (aa) sequence identity with mouse cIAP2.
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAK
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
• Reference: 1. "Entrez Gene: BIRC3 baculoviral IAP repeat-containing 3".
  2. Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A, Korneluk RG (February 1996). "Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes". Nature 379 (6563): 349–53.
  3. Rothe M, Pan MG, Henzel WJ, Ayres TM, Goeddel DV (February 1996). "The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins". Cell 83 (7): 1243–52.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Baculoviral IAP repeat-containing protein 3(BIRC3) detection. Tested with WB in Human.

Target Info

• Gene Symbol: BIRC3
• Gene Full Name: baculoviral IAP repeat containing 3
• Alias Symbols: AIP1;API2;apoptosis inhibitor 2;baculoviral IAP repeat-containing protein 3;cellular inhibitor of apoptosis 2;c-IAP2;CIAP2;HAIP1;HIAP1;IAP homolog C;IAP-1;inhibitor of apoptosis protein 1;MALT2;mammalian IAP homolog C;MIHC;RING finger protein 49;RING-type E3 ubiquitin transferase BIRC3;RNF49;TNFR2-TRAF signaling complex protein;TNFR2-TRAF-signaling complex protein 1.
• NCBI Gene Id: 330
• Protein Name: Baculoviral IAP repeat-containing protein 3
• Description of Target: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.
• Uniprot ID: Q13489
• Molecular Weight: 68372 MW