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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for CHFR Antibody (OAEB00917) |
|---|
| Predicted Species Reactivity | Bovine|Human|Mouse|Porcine|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | IHC |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | CHFR |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Peptide Sequence | KFNHICEQTRFKN |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. |
| Reference | * Scolnick DM, Halazonetis TD.; Chfr defines a mitotic stress checkpoint that delays entry into metaphase.; Nature. 2000 Jul 27;406(6794):430-5.; Pubmed 10935642 |
| Gene Symbol | Chfr|CHFR |
|---|---|
| Gene Full Name | checkpoint with forkhead and ring finger domains |
| Alias Symbols | 5730484M20Rik;C230082M18;checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase;E3 ubiquitin-protein ligase CHFR;RING finger protein 196;RING-type E3 ubiquitin transferase CHFR;RNF1;RNF116;RNF196. |
| NCBI Gene Id | 231600|288734|55743 |
| Protein Name | E3 ubiquitin-protein ligase CHFR |
| Uniprot ID | Q96EP1 |
| Protein Accession # | NP_001154816.1 |
| Molecular Weight | 73.4; 73.3; 72.0; 69.2; 63.9 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CHFR Antibody (OAEB00917)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Porcine|Rat".
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How long will it take to receive "CHFR Antibody (OAEB00917)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CHFR Antibody (OAEB00917)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CHFR Antibody (OAEB00917)"?
This target may also be called "5730484M20Rik;C230082M18;checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase;E3 ubiquitin-protein ligase CHFR;RING finger protein 196;RING-type E3 ubiquitin transferase CHFR;RNF1;RNF116;RNF196." in publications.
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What is the shipping cost for "CHFR Antibody (OAEB00917)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CHFR Antibody (OAEB00917)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CHFR Antibody (OAEB00917)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73.4; 73.3; 72.0; 69.2; 63.9".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CHFR Antibody (OAEB00917)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Chfr|CHFR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Chfr|CHFR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Chfr|CHFR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Chfr|CHFR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Chfr|CHFR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Chfr|CHFR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
