Catalog No: OKCD04479
Price: $700.00
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Datasheets/ManualsPrintable datasheet for CER ELISA Kit (Mouse) (OKCD04479)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Serum (n=5)82-96%88-97%98-105%91-99%
EDTA Plasma (n=5)94-105%93-101%84-92%83-101%
Heparin Plasma (n=5)80-95%86-94%83-102%86-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cerberus (CER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cerberus (CER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cerberus (CER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cerberus (CER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)91-9995
EDTA Plasma (n=5)84-9288
Heparin Plasma (n=5)88-9591
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerberus (CER) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerberus (CER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Col12 Microplate96 Wells (12 x 8 Well strips)
Col12 Lyophilized Standard2
100X Col12 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity11.7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCer1
Gene Full Namecerberus 1, DAN family BMP antagonist
Alias SymbolsCe;Cer;cer-;cer-1;cerberus;cerberus 1 homolog;cerberus homolog;cerberus-like protein;cerberus-related protein;cer-l;Cerl;Cerl1;Cerr1.
NCBI Gene Id12622
Protein NameCerberus
Description of TargetCytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak.
Uniprot IDO55233
Protein Accession #NP_034017.1
Nucleotide Accession #NM_009887.2
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