Catalog No: OKCD04479
Size:96 Wells
Price: $700.00
SKU
OKCD04479
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-96%88-97%98-105%91-99%
EDTA Plasma (n=5)94-105%93-101%84-92%83-101%
Heparin Plasma (n=5)80-95%86-94%83-102%86-102%
ELISA Kit PrincipleAviva Systems Biology CER ELISA Kit (Mouse) (OKCD04479) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CER has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CER is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CER captured in the well.
ELISA Kit Range31.25-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)91-9995
EDTA Plasma (n=5)84-9288
Heparin Plasma (n=5)88-9591
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CER. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CER using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Col12 Microplate96 Wells (12 x 8 Well strips)
Col12 Lyophilized Standard2
100X Col12 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids.
Sensitivity11.7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCER
Alias SymbolsCe, Cer, Cerl, cer-, Cerl1, Cerr1, cer-1
NCBI Gene Id12622
Protein Namecerberus
Swissprot IdO55233
Protein Accession #NP_034017.1
Nucleotide Accession #NM_009887.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CER.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CER.
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