CER ELISA Kit (Mouse) (OKCD04479)

Catalog No: OKCD04479

Size:96WELLS

Mouse CER ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-96%	88-97%	98-105%	91-99%
  EDTA Plasma (n=5)	94-105%	93-101%	84-92%	83-101%
  Heparin Plasma (n=5)	80-95%	86-94%	83-102%	86-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cerberus (CER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cerberus (CER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cerberus (CER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cerberus (CER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.25-2000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	91-99	95
  EDTA Plasma (n=5)	84-92	88
  Heparin Plasma (n=5)	88-95	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerberus (CER) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerberus (CER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Col12 Microplate: 96 Wells (12 x 8 Well strips)
  • Col12 Lyophilized Standard: 2
  • 100X Col12 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: 11.7 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cer1
• Gene Full Name: cerberus 1, DAN family BMP antagonist
• Alias Symbols: Ce;Cer;cer-;cer-1;cerberus;cerberus 1 homolog;cerberus homolog;cerberus-like protein;cerberus-related protein;cer-l;Cerl;Cerl1;Cerr1.
• NCBI Gene Id: 12622
• Protein Name: Cerberus
• Description of Target: Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak.
• Uniprot ID: O55233
• Protein Accession #: NP_034017.1
• Nucleotide Accession #: NM_009887.2