Catalog No: OCOA18458
Size:20UG
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| Datasheets/Manuals | Printable datasheet for CEP55 Protein Lysate (OCOA18458) |
|---|
| Predicted Species Reactivity | Homo sapiens |
|---|---|
| Product Format | Lyophilized |
| Host | HEK293T |
| Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| :: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
| Tag | C-MYC/DDK |
| Description | CEP55 HEK293T cell transient overexpression lysate (as WB positive control) |
| Gene Symbol | CEP55 |
|---|---|
| Gene Full Name | centrosomal protein 55 |
| Alias Symbols | C10orf3;cancer/testis antigen 111;centrosomal protein 55kDa;centrosomal protein of 55 kDa;CT111;MARCH;up-regulated in colon cancer 6;URCC6. |
| NCBI Gene Id | 55165 |
| Protein Name | NM_001127182, NP_001120654, Q53EZ4 |
| Description of Target | Centrosomal protein of 55 kDa, centrosomal protein of 55 kDa [Homo sapiens], Homo sapiens centrosomal protein 55 (CEP55), transcript variant 2, mRNA |
| Uniprot ID | https://www.uniprot.org/uniprot/Q53EZ4 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001120654 |
| Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_001127182 |
| Molecular Weight | 54 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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