CENPO Antibody (OAPB00973)

Catalog No: OAPB00973

Size:100UG

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Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. CENPO Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: CENPO antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO. The immunogen is located within amino acids 170 - 220 of CENPO.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CENPO antibody is Catalog # OEPB03089
• Application Info: CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Fukagawa T. Assembly of kinetochore in vertebrate cells. Exp. Cell Res.2004; 296:21-7.;Okada M, Cheeseman IM, Hori T, et al. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat. Cell Biol.2006; 8:446-57.;Hori T, Okada M, Maenaka K, et al. CENP-O class proteins form a stable complex and are required for proper kinetochore function. Mol. Biol. Cell2008; 19:843-54.;Saito A, Muro Y, Sugiura K, et al. CENP-O, a protein localized at the centromere throughout the cell cycle, is a novel target antigen in systemic sclerosis. J. Rheumatol.2009; 36:781-6.

Target Info

• Gene Symbol: CENPO
• Gene Full Name: centromere protein O
• Alias Symbols: CENP-O;centromere protein O;centromeric protein O;ICEN-36;interphase centromere complex protein 36;MCM21R.
• NCBI Gene Id: 79172
• Protein Name: Centromere protein O|centromere protein O isoform 1 [Homo sapiens]
• Description of Target: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex.
• Uniprot ID: Q9BU64
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_077298