CEBPG ELISA Kit (Human) (OKEI00147)

Catalog No: OKEI00147

Size:96 Wells

Human CEBPG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CEBPG ELISA Kit (Human) (OKEI00147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEBPG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CEBPG
• ELISA Kit Range: 3.125 - 200 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CEBPG. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CEBPG using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CEBPG Microplate: 96 Wells (12 x 8 Well strips)
  • CEBPG Lyophilized Standard: 2
  • 100X CEBPG HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 1.875 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CEBPG
• Alias Symbols: GPE1BP, IG/EBP-1
• NCBI Gene Id: 1054
• Protein Name: CCAAT/enhancer-binding protein gamma
• Description of Target: Transcription factor that binds to the promoter and the enhancer regions of target genes. Binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene . Binds to the promoter and the enhancer of the immunoglobulin heavy chain. Binds to GPE1, a cis-acting element in the G-CSF gene promoter.
• Uniprot ID: P53567
• Protein Accession #: NP_001239225
• Nucleotide Accession #: NM_001252296