CEBPA ELISA Kit (Human) (OKEH07459)

Catalog No: OKEH07459

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CEBPA ELISA Kit (Human) (OKEH07459) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEBPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEBPA captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.3% (n = 20)
  Mean Inter-assay CV%: <=5.5% (n = 20)
• ELISA Kit Component:
  • CEBPA Microplate: 96 Wells (12 x 8 Well strips)
  • CEBPA Lyophilized Standard: 2
  • 100X Biotinylated CEBPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Isoform 4: Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
  Subcellular Location: Isoform 4 Nucleus Nucleolus
  Unigene: Hs.76171
  SMR: P49715
  String: 9606.ENSP00000427514
  KEGG: hsa:1050
  MIM: 116897
  Pfam: PF07716
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.065ng/mL
• Specificity: Natural and recombinant Human CCAAT/enhancer-binding protein alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CEBPA
• Gene Full Name: CCAAT enhancer binding protein alpha
• Alias Symbols: C/EBP-alpha;CCAAT/enhancer binding protein (C/EBP), alpha;CCAAT/enhancer-binding protein alpha;CEBP.
• NCBI Gene Id: 1050
• Protein Name: P49715
• Description of Target: CCAAT/enhancer-binding protein alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P49715
• Protein Accession #: NP_001272758.1
• Nucleotide Accession #: NM_001285829.1