CDC42BPA Antibody (OAEB00167)

Catalog No: OAEB00167 (Formerly GWB-432CD7)

Size:100UG

OAEB00167 (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CDC42BPA
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: SGEVRLRQLEQFI-C
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2.
• Reference: * Zhao Y, Loyer P, Li H, Valentine V, Kidd V, Kraft AS.; Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases.; J Biol Chem. 1997 Apr 11;272(15):10013-20.; Pubmed 9092543

Target Info

• Gene Symbol: Cdc42bpa|CDC42BPA
• Gene Full Name: CDC42 binding protein kinase alpha
• Alias Symbols: A930014J19Rik;CDC42 binding protein kinase alpha (DMPK-like);CDC42 binding protein kinase beta;DMPK-l;DMPK-like;Mrck;MRCK alpha;MRCKA;myotonic dystrophy kinase-related CDC42-binding kinase alpha;myotonic dystrophy kinase-related CDC-42-binding kinase-alpha;myotonic dystrophy kinase-related CDC42-binding protein kinase alpha;myotonic dystrophy protein kinase-like alpha;mytonic dystrophy kinase-related Cdc42-binding kinase;Pk428;serine/threonine-protein kinase MRCK alpha;ser-thr protein kinase PK428;Ser-Thr protein kinase related to the myotonic dystrophy protein kinase.
• NCBI Gene Id: 114116|226751|8476
• Protein Name: CDC42-binding protein kinase beta EMBL AAN86031.1
• Uniprot ID: Q8IWQ7
• Protein Accession #: NP_055641.3
• Molecular Weight: 186, 196