CD8A ELISA Kit (Human) (OKEH00678)

Catalog No: OKEH00678

Size:96T

Human CD8A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CD8A ELISA Kit (Human) (OKEH00678) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD8A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD8A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD8A captured in well.
• ELISA Kit Range: 2.81-180ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.5% (n = 20)
  Mean Inter-assay CV%: < 9.5% (n = 20)
• ELISA Kit Component:
  • CD8A Microplate: 96 Wells (12 x 8 Well strips)
  • CD8A Lyophilized Standard: 2
  • 100X Biotinylated CD8A Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 1.2 ng/mL
• Specificity: Natural and recombinant Human T-cell surface glycoprotein CD8 alpha chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD8A
• Gene Full Name: CD8a molecule
• Alias Symbols: CD8;CD8 antigen, alpha polypeptide (p32);Leu2;Leu2 T-lymphocyte antigen;OKT8 T-cell antigen;p32;T cell co-receptor;T8 T-cell antigen;T-cell antigen Leu2;T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2.
• NCBI Gene Id: 925
• Protein Name: P01732
• Description of Target: T-cell surface glycoprotein CD8 alpha chain
• Uniprot ID: https://www.uniprot.org/uniprot/P01732
• Protein Accession #: NP_001139345.1
• Nucleotide Accession #: NM_001145873.1
• Protein Size (# AA): 235
• Molecular Weight: 26 kDa