CD200R1 ELISA Kit (Human) (OKEH08586)

Catalog No: OKEH08586

Size:96T

Human CD200R1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CD200R1 ELISA Kit (Human) (OKEH08586) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD200R1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD200R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD200R1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.4% (n = 20)
  Mean Inter-assay CV%: <=12.1% (n = 20)
• ELISA Kit Component:
  • CD200R1 Microplate: 96 Wells (12 x 8 Well strips)
  • CD200R1 Lyophilized Standard: 2
  • 100X Biotinylated CD200R1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  Subcellular Location: Isoform 3 Secreted
  Unigene: Hs.309158
  SMR: Q8TD46
  KEGG: hsa:131450
  MIM: 607546
  Pfam: PF08205
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.085ng/mL
• Specificity: Natural and recombinant Human Cell surface glycoprotein CD200 receptor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD200R1
• Gene Full Name: CD200 receptor 1
• Alias Symbols: CD200 cell surface glycoprotein receptor;CD200R;cell surface glycoprotein CD200 receptor 1;cell surface glycoprotein OX2 receptor 1;cell surface glycoprotein receptor CD200;HCRTR2;MOX2 receptor;MOX2R;OX2R.
• NCBI Gene Id: 131450
• Protein Name: Q8TD46
• Description of Target: Cell surface glycoprotein CD200 receptor 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q8TD46
• Protein Accession #: NP_620161.1
• Nucleotide Accession #: NM_138806.3