CCL22 Chemi-Luminescent ELISA Kit (Human) (OKCD03745)

Catalog No: OKCD03745

Size:96WELLS

Human CCL22 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-101%	85-92%	90-97%	85-93%
  EDTA Plasma (n=5)	94-104%	85-101%	87-94%	88-96%
  Heparin Plasma (n=5)	97-104%	81-101%	78-95%	98-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine (MDC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Derived Chemokine (MDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Derived Chemokine (MDC) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-102	96
  EDTA Plasma (n=5)	87-95	90
  Heparin Plasma (n=5)	87-96	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL22 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL22 Lyophilized Standard: 2
  • 100X CCL22 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 27.4 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL22
• Gene Full Name: C-C motif chemokine ligand 22
• Alias Symbols: A-152E5.1;ABCD-1;CC chemokine STCP-1;C-C motif chemokine 22;chemokine (C-C motif) ligand 22;DC/B-CK;macrophage-derived chemokine;MDC;MDC(1-69);SCYA22;small inducible cytokine A22;small inducible cytokine subfamily A (Cys-Cys), member 22;Small-inducible cytokine A22;STCP-1;stimulated T cell chemotactic protein 1;Stimulated T-cell chemotactic protein 1.
• NCBI Gene Id: 6367
• Protein Name: C-C motif chemokine 22
• Description of Target: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
• Uniprot ID: O00626
• Protein Accession #: NP_002981
• Nucleotide Accession #: NM_002990