CCL18/PARC ELISA Kit (Human) (OKBB00418)

Catalog No: OKBB00418

Size:96 Wells

Human CCL18/PARC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL18 has been precoated onto 96-well plates. Standards(E.coli, A21-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL18 amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	49	199	346	53	218	362
  SD	1.62	7.36	14.88	3.66	15.7	27.87
  CV%	3.3	3.7	4.3	6.9	7.2	7.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human PARC Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human PARC Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human PARC Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants and serum
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CCL18
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A21-A89
• Description: For quantitative detection of human CCL18 in cell culture supernatants and serum.

Target Info

• Gene Symbol: CCL18
• Gene Full Name: chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• Alias Symbols: Alternative macrophage activation associated CC chemokine 1, Alternative macrophage activation-associated CC chemokine 1, AMAC 1, AMAC-1, AMAC1, C C motif chemokine 18, CC chemokine ligand 18, CC chemokine PARC, CCL18, CCL18(4-69), CCL18_HUMAN, Chemokine (C C motif) ligand 18, Chemokine (C C) dendritic, Chemokine ligand 18, CKb7, DC CK1, DC-CK1, DCCK1, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, MIP 4, MIP-4, MIP4, PARC, Pulmonary and activation regulated chemokine, Pulmonary and activation-regulated chemokine, Small inducible cytokine A18, Small-inducible cytokine A18
• NCBI Gene Id: 6362
• Protein Name: C-C motif chemokine 18
• Uniprot ID: P55774
• Protein Accession #: NP_002979.1
• Nucleotide Accession #: NM_002988.3