CCL11 Chemi-Luminescent ELISA Kit (Human) (OKCD03418)

Catalog No: OKCD03418

Size:96WELLS

Human CCL11 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-98%	79-92%	79-105%	87-95%
  EDTA Plasma (n=5)	90-98%	83-97%	92-99%	80-99%
  Heparin Plasma (n=5)	94-101%	95-104%	80-94%	85-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Eosinophil Chemotactic Factor (ECF) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	95
  EDTA Plasma (n=5)	85-97	93
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL11 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL11 Lyophilized Standard: 2
  • 100X CCL11 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL11
• Gene Full Name: C-C motif chemokine ligand 11
• Alias Symbols: chemokine (C-C motif) ligand 11;eosinophil chemotactic protein;eotaxin;eotaxin-1;SCYA11;small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin).
• NCBI Gene Id: 6356
• Protein Name: C-C motif chemokine
• Description of Target: C-C motif chemokine
• Uniprot ID: Q6I9T4
• Protein Accession #: NP_002977
• Nucleotide Accession #: NM_002986